Team:Groningen/Labwork/14 September 2013
From 2013.igem.org
Claudio
Plates containing BBa_K1085014-S1-S5-S14 and BBa_K1085014-S2-S5-S13 showed around 20 colonies.Colony PCR (NEB Master Mix) was performed on 4 colonies per plate using F_spank_NheI and HM14 primers (annealing temperature 65°C).
The samples were checked on agarose gel 0.8%.
Plates containing pSB1C3-S1-S5-S13 and pSB1C3-S2-S5-S14 showed thousands of colonies.
Colony PCR (NEB Master Mix) was performed on 4 colonies per plate using VF2 and VR primers (annealing temperature 65°C).
The samples were checked on agarose gel 0.8%.
S13 and S14 were ligated into pSB1C3-S1-S5-S5 and pSB1C3-S2-S5-S5, respectively.
S13 and S14 were ligated into pSB1C3-S16-S3-S5 and pSB1C3-S16-S9-S11, respectively.
The ligation products were plated on LB + Cm.