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Team:Groningen/Labwork/17 September 2013
From 2013.igem.org
Claudio
Plate containing the ligation product BBa_K1085014-S1-S5-S14 was screened again.Colony PCR was performed (NEB Master Mix) on 16 colonies using F-spank-NheI and HM14 primers (annealing temperature 65°C).
The PCR samples were checked on agarose gel 0.8%.
One positive colony (14) was inoculated in LB + Amp.
iGEM 2013 Groningen
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