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From 2013.igem.org

Safety Questions

Please describe the chassis organism(s) you will be using for this project. If you will be using more than one chassis organism, provide information on each of them:

• Species: E. Coli(K12)
• Strain: Invitrogen Top10
• Risk group: 1
• Risk group source link: www.absa.org/riskgroups/bacteriasearch.php?genus=Escheria&species=coli
• Disease Risk to Human: Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys.

What is the highest risk group listed

It is 1.

List and describe all new or modified coding regions you will be using in your project. (If you use parts from the 2013 iGEM Distribution without modifying them, you do not need to list those parts.)

1. Asr-RBS Promoter // Source: PCR from Invitrogen E. Coli. Genomic DNA // E.Coli // Risk Group: 1 // Fn: pH Responsive Promoter
2. GadA-RBS Promoter // Source: PCR from Invitrogen E. Coli. Genomic DNA // E.Coli // Risk Group: 1 // Fn: pH Responsive Promoter
3. Lpp-RBS Promoter // Source: Purchased from IDT // E.Coli // Risk Group: 1 // Fn: Constitutive Promoter
4. BBaE0040 // Source: PCR from Kit Plate 5 Well 14K // Aequeora Victoria // Risk Group: 1 // Fn: Fluoresce green light after absorbing blue light

Do the biological materials used in your lab work pose any of the following risks? Please describe.

Risks to the safety and health of team members or others working in the lab?