Template:Kyoto/Notebook/Aug 27
From 2013.igem.org
Aug 27
Restriction Enzyme Digestion
8/21 pT181 attenuator-1(330µg/mL) | EcoRI | SpeI | 10x BufferB | 100x BSA | MilliQ | total |
---|---|---|---|---|---|---|
3.0µL | 0.5µL | 0.5µL | 3.0µL | 0.3µL | 22.7µL | 30µL |
0.6µL | 0µL | 0µL | 1µL | 0.1µL | 8.3µL | 10µL |
8/17 DT-1(188µg/mL) | EcoRI | XbaI | 10x BufferB | 100x BSA | MilliQ | total |
---|---|---|---|---|---|---|
3.0µL | 0.5µL | 0.5µL | 3.0µL | 0.3µL | 22.7µL | 30µL |
1.0µl | 0µL | 0µL | 1µL | 0.1µL | 7.9µL | 10µL |
8/20 Pcon-RBS-luxR-DT-2(344µg/mL) | EcoRI | SpeI | 10x BufferB | 100x BSA | MilliQ | total |
---|---|---|---|---|---|---|
2.9µL | 0.5µL | 0.5µL | 3.0µL | 0.3µL | 22.8µL | 30µL |
0.6µl | 0µL | 0µL | 1µL | 0.1µL | 8.3µL | 10µL |
Electrophoresis
Lane | Sample | Enzyme1 | Enzyme2 |
---|---|---|---|
1 | 100bp ladder | -- | -- |
2 | 8/21 pT181 attenuator | EcoI | SpeI |
3 | 8/21 pT181 atteniator | -- | -- |
4 | 8/17 DT-1 | EcoRI | XbaI |
5 | 8/17 DT-1 | -- | -- |
6 | 8/20 Pcon-RBS-luxR-DT-2 | EcoI | SpeI |
7 | 8/20 Pcon-RBS-luxR-DT-2 | -- | -- |
8 | 100bp ladder | -- | -- |