From 2013.igem.org
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pH
induced response elicited by certain promoters in bacteria may have
major practical applications. The response can be targeted for specific
pH ranges, for example in tracking the anomalies associated with the
gut micro-biota or detecting pH inside a bioreactor. There are only
limited studies reported in the area. In the present project, a genetic
circuit has been created, using the promoters of the acid shock
response gene from E.coli and the F0F1 ATPase operon from C. glutamicum
that produces a pH dependent colour gradient, much like a universal pH
indicator. A mathematical model has been developed to simulate the
experimental findings. The present study will form the basis for
further research in the field of synthetic biology.
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Feel Free to contact us at igemiitdelhi2013 at gmail dot com if you
have queries; requests; suggestions et cetera.
Thanks to iGEM and IIT Delhi,
we had an awesome summer! |
Our
Project was supported by and done by the students
of IIT Delhi, India.
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This
project was done as a part of iGEM:
iGEM Main
Website
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