Template:Kyoto/Notebook/Sep 2
From 2013.igem.org
Contents |
Sep 2
Liquid Culture
Sample | medium |
---|---|
9/1 entA-(Master Plate)-1 | 4mL SOB(+Km) |
37°C
Colony PCR
Sample | base pair |
---|---|
9/1 RBS-lysis2-DT-(1) | 985 |
Ptet-RBS-lacZα-DT-(1) | 765 |
Plac-RBS-lacZα-DT-(1) | 765 |
Plac-RBS-lacZα-DT-(2) | 765 |
PreDenature | Denature | Annealing | Extension | cycle |
---|---|---|---|---|
94°C | 94°C | 55°C | 68°C | -- |
5min | 30s | 30s | 1min | 30cycles |
Restriction Enzyme Digestion
Liquid Culture
Master Plate
Gel Extraction
EDTA Solution
Colony PCR
Master Plate
Liquid Culture
Colony PCR