From 2013.igem.org
Aug 16
8/15
BBa_R0061(Plax)
BBa_I13504(RBS-GFP-DT))(1)
BBa_I13504(RBS-GFP-DT)(2)
BBa_I0462(RBS-luxR-DT)(1)
BBa_I0462(RBS-luxR-DT)(2)
BBa_K117000(lysis1)(1)
BBa_K117000(lysis1)(2)
BBa_K748002(lysis3)(1)
BBa_K748002(lysis3)(2)
BBa_B0015(DT)
BBa_I732020(RBS-laczα-DT)
Colony PCR
A
| Sample | base pair
|
| 8/15 BBa_I13504(RBS-GFP-DT))(1) | 1174
|
| 8/15 BBa_I13504(RBS-GFP-DT)(2) | 1174
|
| 8/15 BBa_I0462(RBS-luxR-DT)(1) | 1174
|
| 8/15 BBa_I0462(RBS-luxR-DT)(2) | 1174
|
| 8/15 BBa_K748002(lysis3) | 1053
|
| negative control | --
|
| PreDenature | Denature | Annealing | Extension | cycle
|
| 94°C | 94°C | 55°C | 68°C | --
|
| 5min | 30s | 30s | 1min | 30cycles
|
| Sample | base pair
|
| 8/15 BBa_R0061(Plax) | 344
|
| 8/15 BBa_K117000(lysis1)(1) | 456
|
| 8/15 BBa_K117000(lysis1)(2) | 456
|
| 8/15 BBa_B0015(DT) | 443
|
| 8/15 BBa_I732020(RBS-laczα-DT) | 703
|
| negative control | --
|
| PreDenature | Denature | Annealing | Extension | cycle
|
| 94°C | 94°C | 55°C | 68°C | --
|
| 5min | 30s | 30s | 40s | 30cycles
|
Master Plate
A
| Number | Use LB plate (+抗生物質)
|
| ʅ（´◔౪◔）ʃ |
|
Transformation
A
| Name | Sample | Competent Cells | Total | Plate
|
| いでんし | なんちゃらµL | ごにょごにょµL | ほにゃららµL | こーせーぶっしつ
|
| いかどうよう | | | |
|
| | | | |
|
Liquid Culture
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