"
Template:Kyoto/Notebook/Aug 17
From 2013.igem.org
Contents |
Aug 17
Colony PCR
| Sample | base pair |
|---|---|
| 8/15 BBa_K112806(lysis2) -(1) | 828 |
| negative control | -- |
| PreDenature | Denature | Annealing | Extension | cycle |
|---|---|---|---|---|
| 94°C | 94°C | 55°C | 68°C | -- |
| 5min | 30s | 30s | 1min | 30cycles |
| Sample | base pair |
|---|---|
| 8/10 BBa_Q0400 -(1) | 3995 |
| negative control | -- |
| PreDenature | Denature | Annealing | Extension | cycle |
|---|---|---|---|---|
| 94°C | 94°C | 55°C | 68°C | -- |
| 5min | 30s | 30s | 30s | 30cycles |
Liquid Culture
| Sample | medium |
|---|---|
| 8/15 BBa_K112806(lysis2) -(1) | Plusgrow medium(+CP) |
| 8/15 BBa_Q04400(tetR) -(1) | Plusgrow medium(+Amp) |
Master Plate
| Number | Use LB plate (+CP) |
|---|---|
| 1 | BBa_K112806(lysis2) -(1) |
| Number | Use LB plate (+Kana) |
|---|---|
| 1 | BBa_Q04400(tetR) -(1) |
