From 2013.igem.org
Sep 12
Miniprep
Nakamoto
| DNA | concentration[µg/mL] | 260/280 | 260/230
|
| Pcon-pT181atte-DT | 293.6 | 1.88 | 1.84
|
| Pcon-apt12-1R-DT | 303.8 | 1.75 | 1.64
|
| Fusion1atte(pSB1c3) | 172.0 | 1.96 | 2.31
|
| RBS-lisis1-DT | 235.1 | 1.59 | 1.60
|
| RBS-lisis2-DT | 387.7 | 1.91 | 2.01
|
| RBS-lisis3-DT | 387.9 | 1.91 | 2.09
|
| Plux | 166.7 | 1.98 | 1.63
|
Electrophoresis
Nakamoto
| Lane | Sample
|
| 1 | 100bp ladder
|
| 2 | 9/11 P-RBS-lisis1-DT(Colony PCR prodution)
|
File:Igku xxxxxx.xxx
Liquid Culture
Nakamoto
| Sample | medium
|
| 9/10 Pcon-apt12-1R-DT1 | Plusgrow medium(+Amp)
|
| 9/10 DT-Pcon-pT181atte1 | Plusgrow medium(+CP)
|
| 9/10 Fusion1-atte(pSB1c3) | Plusgrow medium(+CP)
|
v>
Electrophoresis
Tatsui
1
| Lane | Sample
|
| 1 | 1kb ladder
|
| 2 | pSB4K5(EcoR1&Spe1)
|
| 3 | pSB4K5 NC(EcoR1&Spe1)
|
| 4 | RBS-lacz-DT(EcoR1&Spe1)
|
| 5 | EcoR1&Spe1 NC( EcoR1&Spe1)
|
| 6 | Pcon-RBS-lacz-DT( EcoR1&Spe1)
|
| 7 | Pcon-RBS-lacz-DT NC( EcoR1&Spe1)
|
| 8 | 1kb ladder
|
File:Igku xxxxxx.xxx
2
| Lane | Sample
|
| 1 | 1kb ladder
|
| 2 | Ptet-pT181anti(Spe1&Pst1)
|
| 3 | Ptet-pT181anti NC(Spe1&Pst1)
|
| 4 | RBS-lysis2-DT(Xba1&Pst1)
|
| 5 | EcoR1&Spe1 NC( EcoR1&Spe1)
|
| 6 | --
|
| 7 | --
|
| 8 | 1kb ladder
|
File:Igku xxxxxx.xxx
Tatsui
| Lane | DNA | Enzyme
|
| 1 | 1kb ladder | --
|
| 2 | RBS-lacZ-DT | EcoR1&Spe1
|
| 3 | RBS-lacZ-DT | EcoR1&Spe1
|
| 4 | RBS-lacZ-DT | EcoR1&Spe1
|
| 5 | -- | --
|
| 6 | Pcon-RBS-lacZ-DT | EcoR1&Spe1
|
| 7 | Pcon-RBS-lacZ-DT | EcoR1&Spe1
|
| 8 | Pcon-RBS-lacZ-DT | EcoR1&Spe1
|
| 9 | 1kb ladder | --
|
File:Igku Aug19electrophoresis3
File:Igku Aug19electrophoresis4
| Name | concentration[µg/mL] | 260/280 | 260/230
|
| RBS-lacZ-DT(EcoR1&Spe1) | 9.8 | 1.50 | 0.39
|
| Pcon-RBS-lacZ-DT(EcoR1&Spe1) | 11.2 | 1.47 | 0.08
|
"
