Template:Kyoto/Notebook/Sep 8

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Contents

1 Sep 8

Sep 8

Gel Extraction

じっけんしゃ

Lane	DNA	Enzyme
1	100bp ladder
2	RBS-lysis1-DT	X+P
3	RBS-lysis1-DT	X+P
5	RBS-lysis3-DT	X+P
6	RBS-lysis3-DT	X+P

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Name	quantity	concentration[µg/mL]	260/280	260/230
（´ω｀）
（ΦωΦ^）

Lane	DNA	Enzyme
1	100bp ladder
3	Pcun-pT181 atte	E+S
4	Pcun-pT181 atte	E+S
6	Spinach-DT	X+P
7	Spinach-DT	X+P
9	RBS-lacZα-DT	X+P
10	RBS-lacZα-DT	X+P

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Name	quantity	concentration[µg/mL]	260/280	260/230
（´ω｀）
（ΦωΦ^）

Lane	DNA	Enzyme
1	1kbp ladder
2	Ptet-anti	E+P
3	Ptet-anti	E+P
5	pSB4K5	E+S
6	pSB4K5	E+S

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Name	quantity	concentration[µg/mL]	260/280	260/230
（´ω｀）
（ΦωΦ^）

Lane	DNA	Enzyme
1	1kbp ladder
2	Pcun-pT181 atte	S+P
3	Pcun-pT181 atte	S+P
5	Plac	S+P
6	Plac	S+P
8	pSB1C3	E+S
9	pSB1C3	E+S
11	pSB1C3	X+P
12	pSB1C3	X+P

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Name	quantity	concentration[µg/mL]	260/280	260/230
（´ω｀）
（ΦωΦ^）

Ligation

じっけんしゃ

state	Vector		Inserter		Ligation High ver.2
experiment	name	xx µg/mL	name	xx µg/mL	xx µg/mL
NC	name	xx µg/mL	name	xx µg/mL	xx µg/mL

Restriction Enzyme Digestion

じっけんしゃ

	DNA	restricion enzyme	restriction enzyme	Buffer	BSA	MilliQ	total
2 cuts	（°°） µL	µL	µL	µL	µL	µL	µL
NC	µL	µL	µL	µL	µL	µL	µL

じっけんしゃ

	DNA	restricion enzyme	restriction enzyme	Buffer	BSA	MilliQ	total
2 cuts	（°°） µL	µL	µL	µL	µL	µL	µL
NC	µL	µL	µL	µL	µL	µL	µL

Transformation

じっけんしゃ

Name	Sample	Competent Cells	Total	Plate
いでんし	なんちゃらµL	ごにょごにょµL	ほにゃららµL	こーせーぶっしつ
いかどうよう

Electrophoresis

じっけんしゃ

Lane	Sample	Enzyme1	Enzyme2
（°Д°）

Lane	Sample	Enzyme1	Enzyme2
（°Д°）

Lane	Sample	Enzyme1	Enzyme2
（°Д°）

Gel Extraction

じっけんしゃ

Lane	DNA	Enzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Name	quantity	concentration[µg/mL]	260/280	260/230
（´ω｀）
（ΦωΦ^）

Lane	DNA	Enzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Name	quantity	concentration[µg/mL]	260/280	260/230
（´ω｀）
（ΦωΦ^）

Lane	DNA	Enzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Name	quantity	concentration[µg/mL]	260/280	260/230
（´ω｀）
（ΦωΦ^）

Lane	DNA	Enzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Name	quantity	concentration[µg/mL]	260/280	260/230
（´ω｀）
（ΦωΦ^）

Lane	DNA	Enzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Name	quantity	concentration[µg/mL]	260/280	260/230
（´ω｀）
（ΦωΦ^）

Ligation

じっけんしゃ

state	Vector		Inserter		Ligation High ver.2
experiment	name	xx µg/mL	name	xx µg/mL	xx µg/mL
NC	name	xx µg/mL	name	xx µg/mL	xx µg/mL

state	Vector		Inserter		Ligation High ver.2
experiment	name	xx µg/mL	name	xx µg/mL	xx µg/mL
NC	name	xx µg/mL	name	xx µg/mL	xx µg/mL

PCR

No name

genome DNA	KOD plus	10x buffer	dNTP	MgSO4	SasA_fwd primer	SasA_rev primer	MilliQ	total
	0.5	2.5	2.5	1.5	0.75	0.75		25

PreDenature	Denature	Annealing	Extension	cycle
°C	°C	°C	°C	--
（◎Д◎）

Gel Extraction

じっけんしゃ

Lane	DNA	Enzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Name	quantity	concentration[µg/mL]	260/280	260/230
（´ω｀）
（ΦωΦ^）

Lane	DNA	Enzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Name	quantity	concentration[µg/mL]	260/280	260/230
（´ω｀）
（ΦωΦ^）

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