From 2013.igem.org
Sep 8
じっけんしゃ
| Lane | DNA | Enzyme
|
| 1 | 100bp ladder |
|
| 2 | RBS-lysis1-DT | X+P
|
| 3 | RBS-lysis1-DT | X+P
|
| 5 | RBS-lysis3-DT | X+P
|
| 6 | RBS-lysis3-DT | X+P
|
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230
|
| RBS-lysis1-DT(X+P) | | 5.3 | 1.98 | 0.06
|
| RBS-lysis3-DT(X+P) | | 5.3 | 1.98 | 0.06
|
| Lane | DNA | Enzyme
|
| 1 | 100bp ladder |
|
| 3 | Pcun-pT181 atte | E+S
|
| 4 | Pcun-pT181 atte | E+S
|
| 6 | Spinach-DT | X+P
|
| 7 | Spinach-DT | X+P
|
| 9 | RBS-lacZα-DT | X+P
|
| 10 | RBS-lacZα-DT | X+P
|
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230
|
| Pcun-pT181 atte(E+S) | | 5.2 | 1.82 | 0.36
|
| Spinach-DT(X+P) | | 8.0 | 1.87 | 0.25
|
| RBS-lacZα-DT(X+P) | | 10.3 | 1.71 | 0.54
|
| Lane | DNA | Enzyme
|
| 1 | 1kbp ladder |
|
| 2 | Ptet-pT181-anti | E+P
|
| 3 | Ptet-pT181-anti | E+P
|
| 5 | pSB4K5 | E+S
|
| 6 | pSB4K5 | E+S
|
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230
|
| Ptet-pT181-anti(E+P) | | 30.6 | 1.84 | 1.16
|
| pSB4K5(E+S) | | 21.8 | 1.87 | 0.98
|
| Lane | DNA | Enzyme
|
| 1 | 1kbp ladder |
|
| 2 | Pcun-pT181 atte | S+P
|
| 3 | Pcun-pT181 atte | S+P
|
| 5 | Plac | S+P
|
| 6 | Plac | S+P
|
| 8 | pSB1C3 | E+S
|
| 9 | pSB1C3 | E+S
|
| 11 | pSB1C3 | X+P
|
| 12 | pSB1C3 | X+P
|
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230
|
| Pcun-pT181 atte(S+P) | | 14.5 | 1.91 | 1.09
|
| Plac(S+P) | | 11.6 | 1.74 | 1.19
|
| pSB1C3(E+S) | | 5.7 | 1.74 | 0.34
|
| pSB1C3(X+P) | | 5.6 | 2.00 | 0.37
|
Miniprep
No name
| DNA | concentration[µg/mL] | 260/280 | 260/230
|
| (・▽・) | | |
|
Ligation
じっけんしゃ
| state | Vector | Inserter | Ligation High ver.2
|
| experiment | name | xx µg/mL | name | xx µg/mL | xx µg/mL
|
| NC | name | xx µg/mL | name | xx µg/mL | xx µg/mL
|
Restriction Enzyme Digestion
じっけんしゃ
| | DNA | restricion enzyme | restriction enzyme | Buffer | BSA | MilliQ | total
|
| 2 cuts | （°°） µL | µL | µL | µL | µL | µL | µL
|
| NC | µL | µL | µL | µL | µL | µL | µL
|
じっけんしゃ
| | DNA | restricion enzyme | restriction enzyme | Buffer | BSA | MilliQ | total
|
| 2 cuts | （°°） µL | µL | µL | µL | µL | µL | µL
|
| NC | µL | µL | µL | µL | µL | µL | µL
|
Transformation
じっけんしゃ
| Name | Sample | Competent Cells | Total | Plate
|
| いでんし | なんちゃらµL | ごにょごにょµL | ほにゃららµL | こーせーぶっしつ
|
| いかどうよう | | | |
|
| | | | |
|
Electrophoresis
じっけんしゃ
| Lane | Sample | Enzyme1 | Enzyme2
|
| （°Д°） | | |
|
| Lane | Sample | Enzyme1 | Enzyme2
|
| （°Д°） | | |
|
| Lane | Sample | Enzyme1 | Enzyme2
|
| （°Д°） | | |
|
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