Template:Kyoto/Notebook/Aug 15
From 2013.igem.org
Contents |
Aug 15
Liquid Culture
Sample | medium |
---|---|
8/10 BBa_B0034(1) | LB (+Amp) |
8/10 BBa_B0034(2) | LB (+Amp) |
8/10 BBa_R0011(1) | LB (+CP) |
8/10 BBa_R0011(2) | LB (+CP) |
8/10 BBa_P0412(1) | LB (+CP) |
8/10 BBa_P0412(2) | LB (+CP) |
Transformation
Name | Sample | Competent Cells | Total | Plate |
---|---|---|---|---|
BBa_I0500(Pbad/araC) | 2 | 17 | 19 | LB (+Kan) |
BBa_F1610(RBS-luxI-DT) | 2 | 17 | 19 | LB (+Amp) |
BBa_R0061(Plux) | 2 | 17 | 19 | LB (+CP) |
BBa_I13504(RBS-GFP-DT) | 2 | 17 | 19 | LB (+CP) |
BBa_I0462(RBS-luxR-DT) | 2 | 17 | 19 | LB (+Amp) |
BBa_K117000(lysis1) | 2 | 17 | 19 | LB (+CP) |
BBa_K112806(lysis2) | 2 | 17 | 19 | LB (+CP) |
BBa_K748002(lysis3) | 2 | 17 | 19 | LB (+CP) |
BBa_B0015(DT) | 2 | 17 | 19 | LB (+CP) |
BBa_Q04400(RBS-tetR-DT-Ptet) | 2 | 17 | 19 | LB (+Kan) |
BBa_I732020(RBS-lacZα-DT) | 2 | 17 | 19 | LB (+CP) |
BBa_J23100 | 2 | 17 | 19 | LB (+Amp) |
Miniprep
DNA | concentration[µg/mL] | 260/280 | 260/230 |
---|---|---|---|
BBa_B0034(1) | 84.1 | 2.11 | 2.01 |
BBa_B0034(2) | 86.9 | 2.03 | 2.02 |
BBa_R0011(1) | 78.3 | 2.26 | 2.02 |
BBa_R0011(2) | 88.9 | 2.24 | 2.02 |
BBa_P0412(1) | 15.61 | 2.10 | 1.93 |
BBa_P0412(2) | 12.93 | 2.18 | 1.91 |