Template:Kyoto/Notebook/Aug 15

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Contents

Aug 15

Liquid Culture

Nakamoto

Samplemedium
8/10 BBa_B0034(1)LB (+Amp)
8/10 BBa_B0034(2)LB (+Amp)
8/10 BBa_R0011(1)LB (+CP)
8/10 BBa_R0011(2)LB (+CP)
8/10 BBa_P0412(1)LB (+CP)
8/10 BBa_P0412(2)LB (+CP)

Transformation

Nakamoto

NameSampleCompetent CellsTotalPlate
BBa_I0500(Pbad/araC)21719LB (+Kan)
BBa_F1610(RBS-luxI-DT)21719LB (+Amp)
BBa_R0061(Plux)21719LB (+CP)
BBa_I13504(RBS-GFP-DT)21719LB (+CP)
BBa_I0462(RBS-luxR-DT)21719LB (+Amp)
BBa_K117000(lysis1)21719LB (+CP)
BBa_K112806(lysis2)21719LB (+CP)
BBa_K748002(lysis3)21719LB (+CP)
BBa_B0015(DT)21719LB (+CP)
BBa_Q04400(RBS-tetR-DT-Ptet)21719LB (+Kan)
BBa_I732020(RBS-lacZα-DT)21719LB (+CP)
BBa_J2310021719LB (+Amp)

Miniprep

No name

DNAconcentration[µg/mL]260/280260/230
BBa_B0034(1)84.1 2.11 2.01
BBa_B0034(2)86.9 2.03 2.02
BBa_R0011(1)78.3 2.26 2.02
BBa_R0011(2)88.9 2.24 2.02
BBa_P0412(1)15.61 2.10 1.93
BBa_P0412(2)12.93 2.18 1.91