Template:Kyoto/Notebook/Sep 18

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Contents

Sep 18

Restriction Enzyme Digestion

No name

9/18Plux SpelPtslBufferBSAMilliQtotal
2cuts3.2µL1µL1µL3µL3µL18.8µL 30µL
NC1.7µL0µL0µL1µL1µL6.3µL 10µL
9/18Pcon-pT181antisense-spin-DT②XbalPtslBufferBSAMilliQtotal
2cuts7.5µL1µL1µL3µL3µL14.5µL 30µL
NC1.4icro;L0µL0µL1µL1µL6.6µL 10µL
9/16 Plac②SpelPtslBufferBSAMilliQtotal
2cuts13.8µL1µL1µL3µL3µL14.5µL 30µL
NC0.7µL0µL0µL1µL1µL7.3µL 10µL
9/16 Spinach-DTXbalPtslBufferBSAMilliQtotal
2cuts17.6µL1µL1µL3µL3µL4.4µL 30µL
NC0.9µL0µL0µL1µL1µL7.1µL 10µL
9/8 Pcon J23100SpelPtslBufferBSAMilliQtotal
2cuts5.7µL1µL1µL3µL3µL16.3µL 30µL
NC0.3µL0µL0µL1µL1µL7.7µL 10µL
9/16 RBS-lacZα-DTXbalPtslBufferBSAMilliQtotal
2cuts8.4µL1µL1µL3µL3µL13.6µL 30µL
NC0.4µL0µL0µL1µL1µL7.6µL 10µL
9/11 Pcon-pT181antisense-spin-DT&#9312:XbalPtslBufferBSAMilliQtotal
2cuts7.2µL1µL1µL3µL3µL14.8µL 30µL
NC0.4µL0µL0µL1µL1µL7.6µL 10µL
9/6 apt12-1R-DTXbalPtslBufferBSAMilliQtotal
2cuts7.9µL1µL1µL3µL3µL14.1µL 30µL
NC0.4µL0µL0µL1µL1µL7.6µL 10µL
9/3 pT181antisenseXbalPtslBufferBSAMilliQtotal
2cuts6.3µL1µL1µL3µL3µL15.7µL 30µL
NC0.3µL0µL0µL1µL1µL7.7µL 10µL
9/14 pSB4K5EcoR1SpelBufferBSAMilliQtotal
2cuts7.3µL1µL1µL3µL3µL14.7µL 30µL
NC0.4µL0µL0µL1µL1µL7.6µL 10µL
8/31 Plux-RBS-GFP-DT EcoR1SpelBufferBSAMilliQtotal
2cuts9.2µL1µL1µL3µL3µL12.8µL 30µL
NC0.5µL0µL0µL1µL1µL7.5µL 10µL
9/12 RBS-lysis3-DT XbalPtslBufferBSAMilliQtotal
2cuts5.2µL1µL1µL3µL3µL16.8µL 30µL
NC0.3µL0µL0µL1µL1µL7.6µL 10µL
9/18 Pcon-pT181attenuatorEcoR1SpelBufferBSAMilliQtotal
2cuts14.3µL1µL1µL3µL3µL7.7µL 30µL
NC0.7µL0µL0µL1µL1µL7.3µL 10µL

Electrophoresis

No name

LaneSampleEnzyme1Enzyme2
1Plae② SpelPtsl
2RBS-lysis3-DTXbalPtsl
3Plux-RBS-GFP-DTEcoR1Spel
4PSB4K5EcoR1Spel
5PconSpelPstl
6PluxSpelPstl
71kbp ladder----
8Plae② ----
9RBS-lysis3-DT----
10Plux-RBS-GFP-DT----
11PSB4K5----
12Pcon----
13Plux----

File:Igku xxxxxx.xxx

Electrophoresis

Okazaki

LaneSampleEnzyme1Enzyme2
1100bp ladder -- --
2Pcon pT181antisense spinach-DT②XbalPstl
3Spinach-DTXbalPstl
4RBS-lacZα-DT XbalPstl
5Pcon pT181antisense-Spinach-DT① XbalPstl
6apt12-1R-DTXbalPstl
7pT181attenuatorXbalPstl
8Pcon attenuatorEcoR1Spel
LaneSampleEnzyme1Enzyme2
1100bp ladder -- --
2Pcon pT181antisense spinach-DT②XbalPstl
3Spinach-DTXbalPstl
4RBS-lacZα-DT XbalPstl
5Pcon pT181antisense-Spinach-DT① XbalPstl
6apt12-1R-DTXbalPstl
7pT181attenuatorXbalPstl
8Pcon attenuatorEcoR1Spel
LaneSampleEnzyme1Enzyme2
1100bp ladder -- --
2Pcon pT181antisense spinach-DT②----
3Spinach-DT----
4RBS-lacZα-DT----
5Pcon pT181antisense-Spinach-DT①----
6apt12-1R-DT----
7pT181attenuator----
8Pcon attenuator---- 
File:Igku xxxxxx.xxx

Gel Extraction

No name

LaneDNAEnzyme
11kbp ldder--
2Plac②Spel+Pstl
3Plac②Spel+Pstl
4----
5RBS-lysis3-DTXbal+Pstl
6RBS-lysis3-DTXbal+Pstl
7----
8Plux-RBS-GFP-DTEcoR1+Spel
9Plux-RBS-GFP-DTEcoR1+Spel
10----
PSB4KSEcoR1+Spel
PSB4KSEcoR1+Spel

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

LaneDNAEnzyme
11kbp ldder--
2PconSpel+Pstl
3 PconSpel+Pstl 4----
5PluxSpel+PStl
6 PluxSpel+PStl
LaneDNAEnzyme
1Pcon PT181 antisense spinach-DTXbal+Pstl
2 Pcon PT181 antisense Spinach-DTXbal+Pstl
3100bp ladder -- 4Pcon PT181 antisense Spinach-DTXbal+Pstl
5 Pcon PT181 antisense Spinach-DTXbal+Pstl
6 1kbp ladder--
LaneDNAEnzyme
1100bp ladder --
2apt12-1R-DTXbal+Pstl
3apt12-1R-DTXbal+Pstl 4----
5PT181 attenuatorXbal+Pstl
6PT181 attenuatorXbal+Pstl
LaneDNAEnzyme
1100bp ladder --
2Pcoa attenuatorEcoR1+Pstl
3Pcoa attenuatorEcoR1+Pstl 4----
5RBS-lacZα-DTXbal+Pstl
6RBS-lacZα-DT Xbal+Pstl
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