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Team:XMU-China/Content7
From 2013.igem.org
- Researcher safety
- Laboratory safety
- Public safety
- Environmental safety
Researcher safety
Every member of our XMU-China 2013 team was trained by our graduate students on exact experimental skills and some laboratory regulations, especially knowledge on safety, including theoretical and practical parts about general lab safety, biosafety and hazardous chemical spill or exposure response. What's more, every member's experiments performing was corrected by our team leader for a period of time, which improves our safety consciousness.
In addition, every member had to read experimental protocol carefully before the experiment. When doing experiments, everyone is demanded to wear gloves to avoid contacting with poisonous chemicals.
Laboratory safety
The materials used in our project pose no safety or health concerns to members in the laboratory. In our experiments we have only two kinds of harmful reagents, both used in SDS-PAGE:
TEMED(N,N,N',N'-Tetramethylethylenediamine): harmful to neural system
Acrylamide: strongly carcinogenic
Considering safety for our researchers and avoiding its harm to the lab and public, some special care and notices were taken. All toxic chemicals are stored at special places separately. Procedures which demanded the protection from contaminations and toxicity are performed on a clean bench. Every toxic reagents would be put back to their original places immediately.
The listed organisms we use (or from which we use parts) are all part of the basic biosafety level 1:
E.coli (K 12) DH5a
E.coli (K 12) MG1655
E.coli (B)   BL21
E.coli (B)   BL21 (DE3)
These four kinds of strains are all non-virulent and are commonly used in microbiological experiments. What's more, none of the new BioBrick parts that we constructed this year raise any safety issues.
First
By constructing robust circuits in E.coli, we want to build a gene network capable of synchronizing genetic oscillations in multiple levels. Cells can be synchronized at the colony level via quorum sensing, and a gas-phase redox will be signaling (mainly H2O2) between colonies simultaneously. Two scales of coupling ensured extremely consistent oscillations.
First
By constructing robust circuits in E.coli, we want to build a gene network capable of synchronizing genetic oscillations in multiple levels. Cells can be synchronized at the colony level via quorum sensing, and a gas-phase redox will be signaling (mainly H2O2) between colonies simultaneously. Two scales of coupling ensured extremely consistent oscillations.
First
By constructing robust circuits in E.coli, we want to build a gene network capable of synchronizing genetic oscillations in multiple levels. Cells can be synchronized at the colony level via quorum sensing, and a gas-phase redox will be signaling (mainly H2O2) between colonies simultaneously. Two scales of coupling ensured extremely consistent oscillations.
