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Template:Kyoto/Notebook/Sep 21
From 2013.igem.org
Contents |
Sep 21
Electrophoresis
| Lane | Sample | Enzyme1 | Enzyme2 |
|---|---|---|---|
| 1 | Pcon-RBS-lacZα-DT | -- | -- |
| 2 | 1kbp ladder | -- | -- |
| 3 | pSB1C3+pT181 attenuator | -- | -- |
Liquid Culture
| Sample | medium |
|---|---|
| 9/2 spinach-DT-2 | -- |
| 8/16 Plux-4 | -- |
| 8/27 Plux-RBS-GFP-DT-1 | -- |
| 9/5 Pcon-pT181 attenuator-1 | -- |
Master Plate
| Number | Use LB plate |
|---|---|
| 1 | 8/27 Pλ-luxI-1(+Amp) |
| 2 | 9/10 Plac(BBa-R0011)-3(+Amp) |
| 3 | 9/5 Pcon-RBS-tetR-DT-1(+Amp) |
| 4 | 9/5 Pcon-pT181 antisense-1(+Amp) |
| 5 | 9/15 Pcon-pT181 attenuator(+Amp) |
| 6 | 9/- Pcon-apt12_1R-DT-1(+Amp) |
| 7 | 9/5 Pcon-spinach-DT-3(+Amp) |
| 8 | 8/27 Pcon-RBS-GFP-DT-Pcon-RBS-luxR-DT(+Amp) |
| Number | Use LB plate |
|---|---|
| 1 | 9/4 pT181 antisense(pSB1C3)-1(+CP) |
| 2 | 9/11 Fusion1 antisense(pSB1C3)-4(+CP) |
| 3 | 9/11 Fusion6 antisense(pSB1C3)-1(+CP) |
| 4 | 9/4 pT181 attenuator(pSB1C3)-1(+CP) |
| 5 | 9/10 Fusion1 attenuator(pSB1C3)-1(+CP) |
| 6 | 9/11 Fusion3n2 attenuator(pSB1C3)-1(+CP) |
| 7 | 9/4 aptamer12_1R(pSB1C3)-1(+CP) |
| 8 | 9/11 aptamer12_P(pSB1C3)-3(+CP) |
| 9 | 9/11 aptamer12_1M(pSB1C3)-1(+CP) |
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| Number | Use LB plate |
|---|---|
| 1 | 8/29 Pbad/araC-RBS-RFP-DT-1(+CP) |
| 2 | 9/4 RBS-lysis3-DT-3(+CP) |
| 3 | 9/10 Pcon-pT181 attenuator-DT-1(+CP) |
| 4 | 9/5 Ptet-pT181 antisense-1(+CP) |
| 5 | 9/4aptamer 12_1R-DT-1(+CP) |
| 6 | 9/5 Ptet-RBS-lacZα-DT-4(+CP) |
| 7 | 8/10 RBS-lacI-DT-2(+CP) |
| 8 | 8/29 Plux-RBS-GFP-DT-1(+CP) |
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