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Template:Kyoto/Notebook/Aug 24
From 2013.igem.org
Contents |
Aug 24
Gel Extraction
| Name | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|
| 8/22 aptamer 12_P (EcoRI & SpeI) | 88 | 21.3 | -- |
| 8/22 aptamer 12_1R (EcoRI & SpeI) | 63 | 23.1 | -- |
| 8/22 aptamer 12_1M (EcoRI & SpeI) | 40 | 22.0 | -- |
| 8/23 Fusion6 antisense (EcoRI & SpeI) | 6.0 | 1.60 | 0.17 |
| 8/23 Fusion6 antisense (XbaI & PstI) | 6.0 | 1.71 | 0.25 |
| 8/23 pT181 antisense (EcoRI & SpeI) | 6.0 | 2.22 | 0.15 |
| 8/23 pT181 antisense (XbaI & PstI) | 6.0 | 2.10 | 0.04 |
| 8/22 Plac (SpeI & PstI) | 6.0 | 1.47 | 0.09 |
| 8/22 Fusion1 antisense (EcoRI & SpeI) | 10 | 2.16 | 0.01 |
| 8/22 J23100 (SpeI & PstI) | 6.0 | 1.52 | 0.35 |
| Name | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|
| 8/22 Spinach (EcoRI & SpeI) | 8.0 | 1.15 | 0.77 |
| 8/22 Fusion6 antisense (XbaI & PstI) | 44 | 1.07 | 0.96 |
| 8/22 Fusion1 antisense (XbaI & PstI) | 2 | -- | 1.00 |
| 8/22 pT181 antisense (XbaI & PstI) | 3. | 2.01 | 0.40 |
| 8/22 pT181 antisense (EcoRI & SpeI) | 8 | 1.13 | 0.82 |
| 8/22 Fusion3m2 attenuator (XbaI & PstI) | 5 | 2.27 | 1.50 |
| 8/22 Fusion3m2 attenuator (EcoRI & SpeI) | 34 | 1.16 | 1.02 |
| 8/22 Fusion1 attenuator (XbaI & PstI) | 14 | 1.40 | 0.62 |
| 8/22 Fusion1 attenuator (EcoRI & SpeI) | 16 | 1.27 | 0.61 |
| 8/22 pT181 attenuator (XbaI & PstI) | 5 | -- | 0.88 |
- These samples were performed ethanol ethanl precipitation.
Restriction Enzyme Digestion
| tetR aptamer 12_P(2) | EcoRI | SpeI | 10x Buffer B | 100x BSA | MilliQ | total | |
|---|---|---|---|---|---|---|---|
| 2 cuts | 2.7µL | 0.5µL | 0.5µL | 3µL | 0.3µL | 23µL | 30µL |
| NC | 0.3µL | 0µL | 0µL | 1µL | 0.1µL | 8.6µL | 10µL |
| tetR aptamer 12_1R(2) | EcoRI | SpeI | 10x Buffer B | 100x BSA | MilliQ | total | |
|---|---|---|---|---|---|---|---|
| 2 cuts | 2.6µL | 0.5µL | 0.5µL | 3µL | 0.3µL | 23.1µL | 30µL |
| NC | 0.3µL | 0µL | 0µL | 1µL | 0.1µL | 8.6µL | 10µL |
| tetR aptamer 12_M(1) | EcoRI | SpeI | 10x Buffer B | 100x BSA | MilliQ | total | |
|---|---|---|---|---|---|---|---|
| 2 cuts | 2.7µL | 0.5µL | 0.5µL | 3µL | 0.3µL | 23µL | 30µL |
| NC | 0.3µL | 0µL | 0µL | 1µL | 0.1µL | 8.6µL | 10µL |
Master Plate
| Number | Use LB plate(+Kan) |
|---|---|
| 1 | entA- #1 |
| 2 | entA- #1 |
| 3 | entA- #1 |
| 4 | entA- #2 |
| 5 | entA- #2 |
| 6 | entA- #2 |
Electrophoresis
| Lane | Sample | Enzyme1 | Enzyme2 |
|---|---|---|---|
| 1 | 100bp ladder | - | - |
| 2 | tetR aptamer 12_P | EcoRI | SpeI |
| 3 | tetR aptamer 12_P | - | - |
| 4 | tetR aptamer 12_1R | EcoRI | SpeI |
| 5 | tetR aptamer 12_1R | - | - |
| 6 | tetR aptamer 12_1M | EcoRI | SpeI |
| 7 | tetR aptamer 12_1M | - | - |
| 8 | 100bp ladder | - | - |
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| Lane | Sample | Enzyme1 | Enzyme2 |
|---|---|---|---|
| 1 | 100bp ladder | - | - |
| 2 | tetR aptamer 12_P | EcoRI | SpeI |
| 3 | tetR aptamer 12_1R | EcoRI | SpeI |
| 4 | tetR aptamer 12_1M | EcoRI | SpeI |
| 5 | 100bp ladder | - | - |
