Team:Groningen/Labwork/26 September 2013

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Mirjam

Checked the plates with MotB+S9+S5 and FliZ+S3+S5. These plates showed only red colonies.
Made a restriction digestion of EstA, S3 and S9 with BamHI. Made a ligation between EstA and the S3 and S9 subunit.

Claudio & Sander

First we now realize that iodine kills Bacillus subtilis.

6 colonies from each Bacillus subtilis transformation plate (BBa_K1085014-BBa_K1085018 and BBa_K1085014-BBa_K1085022) were inoculated in LB + Cm and were streaked out on LB agar + Cm + starch.