Team:NJU China/Project/Brain

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RESULTS

Brain

2013

Liver: For liver targeting, we need to first find a protein specifically recognize hepatic cells. Since Heptitis B virus can infect hepatic cells distinctively, and from recent study[1], we knew that HBV recognizes the hepatic cells via the interaction between the pre-S1 of the HBV envelop protein and NTCP receptor of the hepatic cells. We tried to engineer the pre-S1 from HBV envelope protein to the lamp 2b.
Therefore we cloned the pre-S1 into lamp 2b, and we choose pcDNA 3.1(+) as our backbone.

Results for liver targeting: To produce the exosomes that have pre-S1 on their surface for liver targeting, we first transfected the exosome-producing cells, HEK 293T cells, with the plasmid encoding the fusion protein of lamp 2b and pre-S1 peptide.

Liver

2013

Week2 (14th-18th, April)

siRNA screening (Failed) 14th: We extracted plasmids and cultured the 293t cells. 15th: We transfected plasmids into 293t cells. 16th: We collected cells and preserved it in Trizol. 17th: We extracted RNA, then did RT-PCR with the RNA. 18th: We did qPCR with the cDNA we got on 17th April. 21th: We reexamined the concentration of RNA, and redid qPCR.

Week3 (27th, April – 1st, May)

siRNA screening (Failed) 27th: We cultured the 293t cells in a 12-well plate. 28th: We transfected 293t cells. 29th: We collected cells and extracted RNA from it. 30th: We did RT-qPCR with the RNA we extracted on 29th April. 1st: We did qPCR with the cDNA we got on 30th April.

T cell

2013

Week4 (8th-13th, May)

siRNA screening (Failed), examination whether siRNA are capsulated into exosomes (Success) 8th: We extracted plasmids of 3 kinds of siRNA. 9th: We cultured 293t cells in eight D10 dishes and a 12-well plate. 10th: We extracted 2 kinds of over-expression plasmids and examined the concentration of it. Then we transfect these plasmids into 293t cells respectively. 11th: We collected cells and exosomes from 8 D10 dish, and cells from 12-well plate. 12th: We extracted RNA from cells and exosomes. 13th: We did RT-PCR and qPCR.

Scrollbar Visibility with jScrollPane
Multi-level Photo Map

September 27, 2013 Multi-level Photo Map

Responsive Image Gallery with Thumbnail Carousel

September 20, 2013 Responsive Image Gallery with Thumbnail Carousel

September 12, 2013 Elastislide - A Responsive jQuery Carousel Plugin

Elastislide - A Responsive jQuery Carousel Plugin
Slicebox - A fresh 3D image slider with graceful fallback

September 5, 2013 Slicebox - A fresh 3D image slider with graceful fallback

August

2013

August 30, 2013 Automatic Image Montage with jQuery

Automatic Image Montage with jQuery
Image Zoom Tour with jQuery

August 23, 2013 Image Zoom Tour with jQuery

August 16, 2013 Circular Content Carousel with jQuery

Circular Content Carousel with jQuery
Portfolio Image Navigation with jQuery

August 9, 2013 Portfolio Image Navigation with jQuery

August 4, 2013 Expanding Fullscreen Grid Portfolio

Expanding Fullscreen Grid Portfolio

July

2013

Content Rotator with jQuery

July 29, 2013 Content Rotator with jQuery

July 22, 2013 Vertical Sliding Accordion with jQuery

Vertical Sliding Accordion with jQuery
Animated Text and Icon Menu with jQuery

July 12, 2013 Animated Text and Icon Menu with jQuery

July 5, 2013 Fullscreen Slideshow with HTML5 Audio and jQuery

Fullscreen Slideshow with HTML5 Audio and jQuery
Sliding Background Image Menu with jQuery

July 3, 2013 Sliding Background Image Menu with jQuery

June

2013

June 9, 2013 Grid Navigation Effects with jQuery

Grid Navigation Effects with jQuery

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