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From 2013.igem.org

Light Intensity Test

1. Follow the general protocol to obtain an overnight culture listed above.
2. Follow the protocol to run a 96-well test, setting up the E. colight app as follows, wells are set to a varying intensity from 100% to 0%, decreasing by 20% for every other well. For a clear setup, check the supplemental image. (link and picture relevant to it)
3. Run Fluorescent Analysis Protocol.