Oscillating odorant contains two parts: the Producer and the Oscillator. We choose diacetyl as our desire odorant which widely used as a food additive to emit buttery flavor. In order to enhance the production, we construct the E.coli as the producer . Additionally, we add the oscillation system on the basis of odorant producing system, together, distributing a special volatile—butanedione periodically.
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Receptor aims to transplant a nose for S.cerevisiae to achieve the communication between eukaryotes and prokaryotes through odorant. We select a G protein coupled receptor (GPCR) from C.elegans, odr-10, as our nose to detect the volatile, diacetyl, generated by E.coli. In order to enhance the efficiency of the olfactory receptor, we would modify the downstream pathway by expressing chimeric Gpa1--Gα in yeast and knock out the Far1 gene to disable the cell-cycle arrest, as the optimization.
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