Team:Groningen/26 June 2013

Revision as of 13:33, 26 June 2013 by Mirjam (Talk | contribs)

Extraction of the genomic DNA of B. subtilis strain 168.

Diluting the primers (to 100 mM and 10 mM)
Diluting the dNTPs to a concentration of 10 mM.

Run a PCR for the four different signal sequences.
-FliZ
-MotB
-EstA
-LytB
The size will be around 100-150 bp.

The following protocol is used for the PCR of EstA, MotB and LytB:
98°C, 98°C, 50°C, 72°C, 72°C, 4°C
0:30 0:10 0:25 0:05 10:00 forever

The following protocol is used for the PCR of FliZ:
98°C, 98°C, 45°C, 72°C, 72°C, 4°C
0:30 0:10 0:25 0:04 10:00 forever