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Team:Korea U Seoul/Project/sub design
From 2013.igem.org
Design & Part
We try to express the pearl gene (nacrein) on the E.coli, and check the CaCO3-E.coli structrue. The gene related to nacrein was made by the genetic synthetic technology and go through codon optimization step which for expression of E.coli.
pAT vector is the vector which can express the protein on surface of cell. We made vectors containing 4type of nacrein. The promotor of protien expression was pBAD, so we used arabinose for expression.
Before checking the functioning of Nacrein protein which displayed on cell, purify 4type of nacrein protein and check functioning of them.
| Name | Type | Description | Length | Designer |
|---|---|---|---|---|
| BBa_K114600 | coding |
YfaL_Nacrein(WT) wild type Nacrein, not including the signal sequence. Two carbonic anhydrase(CA) domains and Gly-X-Asn repeat(R) |
2830 | Areum Goh |
| BBa_K114601 | coding |
YfaL_Nacrein CA C-terminal deletion mutant covering one CA domain, not including the signal sequence |
2188 | Areum Goh |
| BBa_K114602 | coding |
YfaL_Nacrein DR The Gly-X-Asn deletion domain. Deleted Repeat(DR) |
2563 | Areum Goh |
| BBa_K114603 | coding |
YfaL_Nacrein R Gly-X-Asn repeat domain(R) |
1807 | Areum Goh |
