Acknowledgement

participating in iGEM is a comprehensive work, which can not be done without the indispensible help from our friends and guiders. Hereby we want to sincerely thanks:

•       Kenji Adzuma, for his patient guiding of the enzymatic modeling.


•       Yancheng Liu, for his marvelous programming and helps in processing data.


•       Prof. David Liu and Vikram Pattanayak, for their generous providing of data for our Cas9 model.


•       Kuanwei Sheng, Jiawei Hang, Wenxiong Zhou, Boxiang Wang, for their devotion in passing their experience and ideas about iGEM to us.


•       Prof. Fenyong Liu and Xiangdong Chen, for their providing of special experiment facilities and resouce.


•       Yao Yang, for his helpful assistance in revising the model.


•       Siqing Wang, Jinhao Zou, Meng Zou, Yupei Wang, Bo Huang, Tengfei Ma, Shimeng Liu, Yun Huang, Liangliang Ji, Wenjia Gu, for their contributions to the brainstorm.


•       Prof. Zhixiong Xie, Prof. Xiangdong Gao, Prof.Yu Chen and College of Life Science, WHU, for their continuous support.

You will not be forgotten.

Attribution

We successfully express the dead cas9(dcas9) BBa_k1081000 in standard backbone pSB1C3 and pSB1A2 in E.coli strain k12 and fused the dcas9 with omega subunit of RNA polymease.

We constructed seven tandem double promoters BBa_k1081002,BBa_k1081003,BBa_k1081004,BBa_k1081005,BBa_k1081006,BBa_k1081007 and BBa_k1081008.

we constructed 7 guide RNA coding sequences and got them sequencced right.

We proved the feasibility of multiple modulation.