Making LB Plates

Prepare Required Solutions

LB Media

Prepare Plates

• Gather sleeves of petri dishes
• Keep the plastic sleeves to place plates into
• Label the petri dishes with date and antibiotic
• Place the plates open in a line when done with labeling

Prepare Solution

• Use 2 L Erlenmeyer Flask for the Agar solution
• Add dry materials and water to flask
• Add magnetic stir bar and stir the media
• Cover the top of the flask loosely with aluminum foil
• Autoclave the media for 30 minutes on a fluid cycle

Pouring the Solution

• Let the solution cool to ~60C

• Go down the line, recapping after each plate is poured
• Use sterile technique
• Fill each plate to about half-full of agar
• If there are bubbles, try to pop them by passing plate over a flame
• Add antibiotics to solution if needed

• Let the plates solidify right side up
• Flip the agar side on top to keep condensation off of the gel when solid
• Leave out on lab bench
• Next day, store the plates in original sleeves in 4C