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Team:DTU-Denmark/Methods
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Revision as of 12:47, 30 July 2013 by
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Home
Team
Project
Description
Parts
Timeline
Notebook
pBAD SPL
Experiments
Modeling
Protocols
Hello World Pilot Project
Safety
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Overview
High School Outreach
IP and Synthetic Biology
BioBrick Workshop
Helping Other Teams
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iGEM
Methods
Contents
1
PCR
2
Plasmid isolation
PCR
PCR
PCR-mix
PCR-ramp
PCR-touchdown
PCR-touchUp
PCR-gradient
Colony PCR
Plasmid isolation
Follow QIAprep Spin Miniprep Kit protocol from steps 1 - 4
Centrifuge for 1h at 4C and 20000 g