Kyoto:projectRNA/futureview

FutureView-assay

Assay

1. Qualitative assay of tetR

inducible tetR aptamer to drive GFP-expression on plate. As negative controls, we will use RNA with antisense, attenuator, spinach, no-RNA and attenuator-tetR aptamer. As positive controls, we will also use GFP. As a consequence, we will expect the colony analyzing X-gal express the blue with &beta-gal, the negative control express the white, and that the positive control do the blue.

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3. Qualitative assay of spinach assay

We will check that DFHBI fluorescence on a plate with spinach. We cultivate IPTG-inducible Spinach in a liquid culture under a shading condition, and add DFHBI. Then we check whether this sample fluorescence after centrifugation. We also check spinach-GFP and antisense-spinach.As a result, we expect the pellet fluorescence just with spinach and DFHBI.

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