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Team:Peking/DataPage/Parts
From 2013.igem.org
Parts
XylS Main Page-BBa_K1031911 XylS is a σ70-dependent transcriptional factor that activates Pm promoter when benzoate acid or its derivative is present. We exploited it to build a XylS biosensor circuit. Adaptor for this biosensor will improve the detection performance of XylS. Visit Wiki
HbpR Main Page-BBa_K1031300 HbpR is a σ54-dependent transcriptional factor, which activates PC promoter when 2-hydroxybiphenol or 2-aminobiphenol is in the presence. We built a HbpR biosensor circuit and fine-tuned its performance in E.coli. Visit Wiki
NahF Main Page-BBa_K1031620 NahF is the salicylaldehyde dehydrogenase to transform salicylaldehyde into salicylic acid (salicylate) using NAD+. We tested NahF in E.coli and showed that the NahF can work as an Adaptor for the NahR biosensor NahR, significantly improving the performance and range of detection. Visit Wiki
Hybrid Promoter Main Page-BBa_K1031021 The hybrid promoter is constructed for the band-pass filter. As for the reporter node, a special promoter is used so that the reporter can be controlled by the activator and inhibitor at the same time. Promoter engineering was performed to serve the purpose. Additionally, we tested this hybrid promoter and constructed a model for it. Visit Wiki
NahR Main Page-BBa_K1031610 Pre-existing Part: Main Page-BBa_J61051 BBa_K1031610 is upgraded from previous part BBa_J61051 designed by Arkin Lab. NahR is a 34 kDa transcription factor mined from Pseudomonas putida which binds to nah and sal promoters to activate transcription in response to the inducer salicylate. We constructed a Psal/NahR biosensor circuit using sfGFP as reporter gene and systematically characterized its performance . Adaptor of this biosensor not only improves detection efficiency but also expands detection profile. Visit Wiki
Parts for Biosensors
DmpR BBa_K1031221 BBa_K1031222 BBa_K1031223 BBa_K1031224 are DmpR biosensor's reporters with different RBS.
HbpR BBa_K1031300 is the coding sequence of HbpR with terminators. BBa_K1031301 and BBa_K1031302 are HbpR biosensor's reporters with different RBS.
HcaR BBa_K1031410 is the coding sequence of HcaR with terminators. BBa_K1031441 BBa_K1031442 BBa_K1031443 and BBa_K1031444 are HbpR transcriptional factors with different constitutive promoters (Pc). BBa_K1031420 and BBa_K1031421 are HcaR biosensor's reporters with different RBS.
CapR BBa_K1031100 is the coding sequence of CapR transcriptional factor. BBa_K1031111 and BBa_K1031112 are basic sensor constructions with different CapR expression level. BBa_K1031113 and BBa_K1031114 are reporters with different RBS for the CapR biosensor.
HpaR BBa_K1031510 is the coding sequence of HpaR transcriptional factor. BBa_K1031520 and BBa_K1031521 are reporters with different RBS for the HpaR biosensor.
NahR BBa_K1031610 is the NahR biosensor circuit.
PaaX BBa_K1031710 is the coding sequence of PaaX with terminators. BBa_K1031742 BBa_K1031743 and BBa_K1031744 are PaaX transcriptional factors with different Pc.
XylS BBa_K1031911 is the coding sequence of XylS transcriptional factor with terminators. BBa_K1031912 BBa_K1031913 and BBa_K1031916 are XylS transcriptional factors with different Pc.
XylR BBa_K1031803 BBa_K1031804 are XylR biosensor's reporters with different RBS.
Parts for Adaptors
NahF BBa_K1031620 is the coding sequence of XylS transcriptional factor with terminators. BBa_K1031621 BBa_K1031622 BBa_K1031623 BBa_K1031624 and BBa_K1031625 are coding sequences of enzyme NahF under different Pc.
Parts for Band-Pass Filter
BBa_K1031021 is the hybrid promoter for the reporter node, which can be activated and inhibited at the same time by two different transcriptional factors. BBa_K1031011 is the transcriptional factor used as the activate node in the band-pass filter. Each BBa_K1031013 BBa_K1031014 BBa_K1031015 BBa_K1031016 is cI with different of RBS and pSal as its promoter, which is a crucial part for band-pass filter.
Figure 1. The aromatics spectrum showing the aromatics-sensing profiles of our individual biosensors. Each color segment in the central spectrum represents the detection profile of a biosensor. Structural formula highlighted in color stand for the aromatic compounds that can be detected by our biosensors.┝ stand for Adaptors, enzymes that convert undetectable compounds into detectable aromatics, thus to expand the detection capacity of some biosensors. Click Here for the summary table of the aromatics spectrum and aromatics-sensing profiles of individual biosensors. Visit Our Wiki
Figure 2. Experimental points and the linear fitting curves of the orthogonality test. The black dashed lines are with the slopes of 1, showing as the reference line. The slopes of the experimental fitting curves were showed in the upside portion of the figure, all of them were around 1. These data showed the orthogonality among inducers of biosensors(a, b) XylS and NahR; (c, d) XylS and HbpR; (e, f) NahR and HbpR, (g, h) XylS and DmpR, (i, j) NahR and DmpR, and (k, l) HbpR and DmpR. The experimental points and linear fitting curves of biosensor and its inducers are marked in different colors: XylS in red, NahR in green, HbpR in orange and DmpR in dark cyan.(For more details, please Click Here)
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