Notebook : June 26

Lab work

- Design of the oligonucleotide for the amplification of Pbphr1 (intergenic sequence upstream) for Pseudomonas pseudoalcaligenes PbphD and KF707 (intergenic sequence upstream) for Burkholderia xenovorans LB400

- 500 ml Buffers (TB: the Transformation Buffer) prepared to make super competent bacteria, stored at 4°C

- 250 ml of culture of E.Coli DH5αZ1 in LB prepared for overnight culture, with an initial Do (Optical Density) of 0.04 at 17h30min

- Protocol of extraction of the genomic DNA from bacteria (E.Coli DH5α) written

- Documentation about the fnr activator operator sequences in iGEM registry

- Team meeting and discussion with Munich team (Skype)