Exeter/8 August 2013

Liquid Cultures, Miniprepped

• [http://parts.igem.org/Part:BBa_K592012 K592012] (Magenta) CAM

• [http://parts.igem.org/Part:BBa_J04450 RFP control] CAM

• [http://parts.igem.org/Part:BBa_R0082 R0082] (ompC promoter) CAM

• DH5α 3 (Tom and Adam's control) TET

• DH5α 5 (Tom and Adam's RBS + Cyan) TET

• DH5α 1 (Tom and Adam's RBS + cph8) TET

• [http://parts.igem.org/Part:BBa_S05058 S05058] (RBS + yellow) CAM

• [http://parts.igem.org/Part:BBa_K592010 K592010] (yellow) CAM

• [http://parts.igem.org/Part:BBa_K592018 K592018] (RBS + cph8) CAM

• [http://parts.igem.org/Part:BBa_K608002?title=Part:BBa_K608002 K608002] (RBS + promoter) CAM

• [http://parts.igem.org/Part:BBa_B0015 B0015] (Terminator) CAM

• [http://parts.igem.org/Part:BBa_K322124 K322124] (RBS + cph8) CAM

( 3 replicates, #2, #3 and #4.)

NanoDropping Promoter + RBS Replicates

1 = 29.6ng/ul

1 = 29.2ng/ul

2 = 12.2ng/ul

2 = 12.5ng/ul

3 = 22.8ng/ul

3 = 23.6ng/ul

4 = 17.8ng/ul

4 = 17.5ng/ul

NanoDrop of liquid cultures

We sent off cph8 K322124 red light sensor for sequencing.

2 replicates each of #4, #3 and #2 from glycerol stocks were done. 10ul in Eppendorfs.

Take me back to the notebook.