Here's our lab log, you can read about what we've been doing in the lab. (We're working hard, promise)
June 2013
Week 1
24th June
We’ve moved into our office, deep in the basement of the research labs in the Miall building.
Our time has been taken up getting acquainted with our office ( and ourcoffee machine!) and roughing out the fine details of our protocol.
We’ve pulled together a combination of the teams various projects to create our final idea, we’re now looking at making a detector platform for physically binding substrates to activate a reporter. (Scintillating no?)
Our team has been busy thinking of inventive assay design to characterise our biobricks; frantically trying to code our wiki and taking our team photos (important work)
25th June
Jeni, one of our first year biochemists has been working on her primer design, getting everything ready to start in the labs next week. We’ve been compiling our part list and getting our order ready; a buffer and bacteria shopping spree.
26th June
We’re trying to plan out our outreach programs, so watch this space. We’re going to be doing instructional videos on RC10 and golden brick assembly; as well as some more basic introduction to Synthetic Biology for younger students, alongside running some SynBio workshops for summer students that’ll be here at Leeds.
27th June
Today we've been working on our presentation; getting it ready for the YSB conference by running it past some of our lecturers; scary stuff. We've also been getting comfortable in the lab space, making up stacks of LB broth and preparing for some serious bacterial colonisation.
28th June
It's been a busy week here at Leeds iGem HQ. We've got a Biochemist and a Physicist (a rather handsome pair) frantically working on coding the wiki and creating images for the presentations and posters, it's gonna be beautiful (we hope)
We've had a team of biochemists (all 3 of them) hard at work designing primers and getting ready to steamroll into the lab next week.
July 2013
Week 2
1st July
With our primers in the post, we've been mass producing growth media and agar plates. We've resuspended and transformed a selection of the biobricks we plan on using, including YFP, GFP, INP, and some terminators.
The wiki team have been locked in the basement and their not allowed out till it looks beautiful.
2nd July
Today we've been writing out scripts for our tutorial videos, they'll be a series aimed at younger students with an introduction to synthetic biology, and a series aimed at Synthetic Biologists on things like Goldengate and Gibson assembly.
Then we locked our token nanotechnologist into a lab with a couple of thousand pounds worth of microfluidics chips and we're hoping for the best.
3rd July
Been a busy day; plasmid preps, glycerol stocks and further transformations of quorum sensing biobricks (pLux and LuxR), promoters and cyan fluorescent proteins.
We then had a skype conference Cyrille Pauthenier, the advisor for the Evry iGem team.
This rather charming fellow went into great detail on Goldengate and Goldenbrick assembly methods and their pros and cons. We ended our day doing soundtests for our educational videos; watch for the remix.
4th July
Another day of plasmid preps and innoculations in the lab of our newly transformed biobricks.
Meanwhile in the office we have been working on our poster for the young synthetic biologist confrence in london next week.
5th July
Today we have done further plasmid preps getting ready for our assembly!
In the office we've been working on our poster and presentation for the upcoming young synthetic biologist confrence in london
There has also been organisation of trips into schools making a presentation for 6th form students on synthetic biology
Finally we have been double checking primers and DNA sequences for gibson assembly
Week 3
8th July
Our first biobrick assembly has been done!(A green Flourescent protein). It will be tested to see if it has worked tomorrow
Presented our ideas so far to two more academic staff.
We have been doing the finishing touches to our poster for the Young Synthetic Biologist confrence in london this weekend
Jonah has been working very hard on our first youtube tutorial video
9th July
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10th July
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11th July
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12th July
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Week 4
15th July
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16th July
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17th July
Meeting with our new sponsors, BioLine
18th July
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19th July
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Week 5
22nd July
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23rd July
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24th July
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25th July
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26th July
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Week 6
29th July
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30th July
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31st July
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August 2013
Week 6
1st August
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2nd August
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Week 7
5th August
Paul starts hiking along Hadrian's Wall for charity!
6th August
Day 2 of hiking for Paul, raising money for the Crohn's and Ulcerative Collitis Unit in the Leeds Teaching Hospitals Trust.
7th August
Day 3 of hiking for Paul. He should be very tired by now.
8th August
Day 4 of hiking for Paul. Nearly done!
9th August
Final Day for Paul hiking along Hadrian's Wall.
Week 8
12th August
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13th August
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14th August
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15th August
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16th August
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Week 9
19th August
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20th August
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21st August
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22nd August
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23rd August
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Week 10
26th August
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27th August
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28th August
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29th August
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30th August
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September 2013
Week 11
2nd September
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3rd September
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4th September
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5th September
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6th September
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Everything Below Here To Be Removed
This is a template page. READ THESE INSTRUCTIONS.
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.
You MUST have all of the pages listed in the menu below with the names specified. PLEASE keep all of your pages within your teams namespace.
You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.
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