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From 2013.igem.org

{ "date" : "2013-07-01", "author" : "emil", "title" : " Re-screening, amplification and digestion(together with the two B. subtilis backbones) of the GFPs E0840 and E0240 ", "content" : "I screened the three other sample of GFP(0840/2,0840/4,0240/1,0240/3) like in the\"> previous post with the following results:(the gel was run together with Girelli's sample, we are interested in the first 4 sample)

We can see 2 bands for each sample: the upper is the plasmid(pSB1A2 2079 bp), the lower is presumably the GFP(BBa_E0240 826 bp,BBa_E0840 828 bp)(prefix and suffix escluded)at 900 bp.Afterwards i have amplified 0.5 6micro;l of one of the 4 sample(E0840/4 475.9 ng/micro;l) following the PCR protocol and exploiting the universal primers(prefix Forward,Suffix Reverse) with the following resuts(as usual I did a gel to verify the pcr): As we can see the pcr completed succesfully, there are the right bands at 900-1000 bp.Then I purified the pcr following the purification protocol . After that I have quantified the product with the following results:Finally I have digested o/n all the 48.5µl of b sample and of 2 sample of the backbone(K823026 359.7ng/µl,K823024 334.4ng/µl) with EcoR1-HF and Pst1 following the digestion protocol. ", "tags" : "K832024-K823026-E0840-E0240" }