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From 2013.igem.org

Labbook |	Protocols |	Materials |

Contents 1 Chloramphenicol 1.1 About 1.2 Stock 1.3 Stability 1.4 References

Chloramphenicol

About

Chloramphenicol is a bacteriostatic antibiotic that specifically binds resudies in the 50S ribosomal subunit, preventing peptide bond formation.^[1]

Stock

1. Mix:
   • 1 g chloramphenicol (25 mg/mL)
   • ad. 40 mL ethanol
2. aliqout in 1 mL stocks and store at -20°C

Stability

• Chloramphenicol is not autoclavable, it has to be added before pouring plates (keep in mind that the agar is not too hot when adding it)

References

http://en.wikipedia.org/wiki/Chloramphenicol