Team:TU Darmstadt/materials/Antarctic Phosphatase

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Contents

Antarctic Phosphatase

About

Antarctic phosphatase catalyzes the removal of 5´ phosphate groups from DNA and RNA. Since phosphatase-treated fragments lack the 5´ phosphoryl termini required by ligases, they cannot self-ligate. This property can be used to decrease the vector background in cloning strategies.[1]

Working conditions

Buffer: 1X Antarctic Phosphatase buffer

Supplier

  • NEB http://www.neb.com/nebecomm/products/productM0289.asp

References

[1] http://www.neb.com/nebecomm/products/productM0289.asp

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