Team:Groningen/protocols/Motility assay

Motility assay

Ad 13 ml of the motility agar (0.4% or 0.7%) per plate.

Inoculate the plates at the center with the Strain of interest (strain A).

For every plate inoculated with Strain A inoculate a plate with Wild type (WT).

inoculations is done with 10 ul of liquid culture with an OD₆₀₀ of 0.4.

Let the colonies grow over 16 hours.

After the 16 hour growth period the measure the spread of the colony every 2 hours.

Motility Agar 0.7% (100 ml)

Motility Agar 0.4% (100 ml)

compound	amount	treatment
LB broth	2 g
agar	0.7 g

compound	amount	treatment
LB broth	2 g
agar	0.4 g