Team:Groningen/Labwork/12 July 2013
From 2013.igem.org
Mirjam Made a PCR with (1) no strep tag (2) strep F (3) strep R. The annealing temperature for (1) and (2) is 78 degrees Celsius. For (3) the annealing temperature is 80 degrees Celsius.
Gel purification of the strep F silk obtained yesterday.
Run a 0.8% agarose gel for 30 min at 90V.