A

• acrylamide – our subject of study

B

• BiFC – Bimolecular Fluorescent Complementation

C

• cloning – on pSB1C3 of course!
• calm resignation – general mood two days prior to wiki freeze (see 'panic attack')
• mCherry - red fluorescent protein. We managed to succesfully clone C- and N-terminal BiFC fragments into pSB1C3 and deliver them to the Registry

D

• despair - general mood week prior to wiki freeze (see 'calm resignation')

E

• ethanol – lots of ethanol in cell lab (take a deep breath ladies and gentelmen - unless one of you is the designated driver)

F

• FluoSafe – our project

G

• Generacja Przyszłości (eng. Generation of the Future) – our grant

H

• HeLa and HEK – cells we worked with
• hemoglobin - core of our sensor

I

• irritation - when this **** C-m6 and C-m12 cannot clone in pSB1C3 plasmid!

J

• Jamboree - we're waiting for it

K

• kill-switch - our safety system which wasn't synthetized on time (see: 'lysis device' and 'MinC')

L

• Lyon - we can send you postcard from jamboree
• lysis device - another component of our kill-switch (see: 'MinC')

M

• MinC - core of our kill-switch. Inhibits bacterial division.

N

• N-terminal valine - acrylamide forms adducts on the N-terminus of hemoglobin alpha and beta chains. Adducts interfere with tetramer formation. We attempted to take advantage of this property while designing the hemoglobin-based acrylamide sensor.

O

• mOrange - we nearly managed to produce BiFC fragments form this fluorescent protein

P

• PCR – polymerase chain reaction
• panic attack – begins approximately 12 h prior to wiki freeze

Q

• questionable - large portion of our clones and PCR products

R

• roGFP - redox-sensitive GFP

S

• superfolder proteins - improved derivatives of A. victoria-based fluorescent proteins (green, yellow, blue and cyan). This part of our project actually succeded. They're brighter, fun to work with and considerably more stable (as a poet once said: 'harder, better, faster, stronger' - and our work with them still isn't over)

T

• Toolbox (BiFC's) - one of our goals

U

• ultraviolet - and GFP glows

V

• vacation - in lab all the time!

W

• wirówka (eng. centrifuge) – that we unintentionally broke!
• wiki freeze – the ultimate deadline (see: "despair', 'calm resignation' and 'panic attack')

X

Y

• yellow fluorescent proteins (sfYFP and SYFP2) - and their glowing rivalry!

Z