Team:Heidelberg/Templates/DelH week24
From 2013.igem.org
23-09 - 29-09-13
Generation of DelH plasmids pFS02, pFS03, pFS04 and pFS05
Amplification of pFS_04 Backbone (FS_85 to FS_94; 4.1 kbp)
- Reaction
Reagent | Amount |
---|---|
pSB6A1 | 1 |
FS_85: (1/10) | 1 |
FS_94: (1/10) | 1 |
Phusion flash Master Mix | 10 |
DMSO | 1 |
dd H2O | 4 |
- Conditions
Biorad T100 | ||
---|---|---|
Cycles | temperature [°C] | Time [s] |
1 | 98 | 10 |
35 | 98 | 1 |
72 | 1:25 | |
1 | 72 | 10min |
1 | 12 | inf |
Results:
Amplification of pFS_02 Backbone (HM_17 to FS_78; 4.1 kbp)
- Reaction
Reagent | Amount |
---|---|
pSB6A1 | 1 |
HM_17: (1/10) | 1 |
FS_78: (1/10) | 1 |
Phusion flash Master Mix | 10 |
DMSO | 1 |
dd H2O | 4 |
- Conditions
Biorad T100 | ||
---|---|---|
Cycles | temperature [°C] | Time [s] |
1 | 98 | 10 |
35 | 98 | 1 |
72 | 1:25 | |
1 | 72 | 10min |
1 | 12 | inf |
Results:
Amplification of pFS_02 Backbone (HM_17 to FS_85; 4.1 kbp)
- Reaction
Reagent | Amount |
---|---|
pSB6A1 | 1 |
HM_17: (1/10) | 1 |
FS_85: (1/10) | 1 |
Phusion flash Master Mix | 10 |
DMSO | 1 |
dd H2O | 4 |
- Conditions
Biorad T100 | ||
---|---|---|
Cycles | temperature [°C] | Time [s] |
1 | 98 | 10 |
35 | 98 | 1 |
72 | 1:25 | |
1 | 72 | 10min |
1 | 12 | inf |
Results:
Amplification of pFS_03 Backbone (FS_86 to FS_87; 4.1 kbp)
- Reaction
Reagent | Amount |
---|---|
pSB4K5 | 1 |
FS_86: (1/10) | 1 |
FS_87: (1/10) | 1 |
Phusion flash Master Mix | 10 |
DMSO | 1 |
dd H2O | 4 |
- Conditions
Biorad T100 | ||
---|---|---|
Cycles | temperature [°C] | Time [s] |
1 | 98 | 10 |
35 | 98 | 1 |
72 | 1:25 | |
1 | 72 | 10min |
1 | 12 | inf |
Results:
Amplification of pFS_02 Backbone (FS_86 to FS_87; 4.1 kbp)
- Reaction
Reagent | Amount |
---|---|
pSB4K5 | 1 |
FS_86: (1/10) | 1 |
FS_87: (1/10) | 1 |
Phusion flash Master Mix | 10 |
DMSO | 1 |
dd H2O | 4 |
- Conditions
Biorad T100 | ||
---|---|---|
Cycles | temperature [°C] | Time [s] |
1 | 98 | 10 |
35 | 98 | 1 |
72 | 1:25 | |
1 | 72 | 10min |
1 | 12 | inf |
Results: