208

PCR of pZE21

Mastermix:

• dNTP 5ul
• HF Buffer 50ul
• polymerase 2.5ul
• water 157.5ul
• fw primer (1A) 15ul
• rv primer 1B 15ul
• temlate 1ul/tube

Transformations

• from plate 3: [http://parts.igem.org/Part:BBa_I0500 I0500]
• from plate 3: [http://parts.igem.org/Part:BBa_K808000 K808000]
• from plate 3: [http://parts.igem.org/Part:BBa_K206001 K206001]
• from plate 3: [http://parts.igem.org/Part:BBa_K564012 K564012]
• from plate 3: [http://parts.igem.org/Part:BBa_I13458 I13458]
• from plate 5: [http://parts.igem.org/Part:BBa_I13458 I13458]

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