Team:Groningen/Labwork/18 July 2013

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Sander
prepared 20 tubes for use in -80 c Glyserol stock

Inne Cells with BBa_k818000, inoculated overnight, were successfully grown. Both negative controls were clear, and the rest of the samples was turbid. Did a miniprep of the samples 1 & 2 (the ones with Amp). Afterwards concentration of DNA was determined via nanodrop.

Results: sample 1 60.1 ng/uL, sample 2 31.5 ng/uL

During the miniprep the filtered LB medium was poured back into the culture (so cells could grow again). A -80 C stock was made with 1 mL culture (sample 1).