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From 2013.igem.org

Sander
prepared 20 tubes for use in -80 c Glyserol stock.
created a restriction digest reaction for silk.

Inne & Mirjam
Cells with BBa_k818000, inoculated overnight, were successfully grown.
Both negative controls were clear, and the rest of the samples was turbid.
Did a miniprep of the samples 1 & 2 (the ones with Amp).

#	composition	Bacteria
1	4mL LB 8uL Amp	+
2	4mL LB 8uL Amp	+
3	4mL LB 8uL Amp (negative control)	-
4	4mL LB	+
5	4mL LB (negative control)	-

Afterwards concentration of DNA was determined via nanodrop.

Results: sample 1 = 60.1 ng/uL, sample 2 = 31.8 ng/uL

During the miniprep the filtered LB medium was poured back into the culture (so cells could grow again).
A -80 C stock was made with 1 mL culture (sample 1). (stored in tray: iGEM 2012 A, compartment 70)