Team:Groningen/Labwork/22 July 2013

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Revision as of 09:01, 22 July 2013 by Mirjam (Talk | contribs)

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Mirjam
Made a PCR for silk 2 and 3 (strepF-R and F-strepR)in triplo
- 28 ul MQ
- 1.5 ul DMSO
- 10 ul GC buffer
- 1 ul of each dNTP
- 2.5 ul primer F
- 2.5 ul primer R
- 0.5 ul phusion
- 1 ul genomic DNA (BBa_...016)

Run a gel with the ligation product of silk and the PCR afterwards. Figured out why the ligation did not work.

Made a new restriction digest for silk without strep tag.