Gel electrophoresis

Materials:

• Power supply
• Agarose gel
• Tray
• DNA samples
• DNA Ladder

Procedure:

All the DNA samples are mixed 1:1 ratio with Loading Dye 2x.
The samples are loaded on a 0.8% or 1.5% agarose gel (diluted in TBE buffer 1x), with next to it a DNA 1kb gene ruler of Fermentas.
A 90V electric potential is applied to the gel for 24-45 minutes to seperate the bands.
The gel is run in TBE buffer 1x.