lab 208

Main purpose

• Colony PCR from Pseudomonas aeruginosa (PAO) to isolate Nir.
• Colony PCR to check the presence of AMO and HAO in transormants E.coli.

Who was in the lab

Kristian, Gosia, Henrike, Julia

Procedure

Colony PCR

Colony PCR was performed according to standard method in order to check if transformed cells which grew on medium contain desired insert in pZA21 plasmid. The colonies were chosen from agar plate to be checked. Names and numbers of colonies as well as plates names and primers are:

• HAO 1-6 in pZA21 USER, primers 18a, 18b
• AMO 1-9 in pZA21 USER, primers 17a, 17b

PCR programms based on standard [http://https://2013.igem.org/Team:DTU-Denmark/Methods/PCR/Generel_example_on_a_normal_PCR-program| PCR programm] with differences in annealing temperatures and extension time as it was done on 12-07-2013.

Results

Conclusion

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