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From 2013.igem.org

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• ZombiE.coli
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• Parts Submitted
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• Attributions

Contents 1 Team University of Texas at Austin 1.1 Who we are 1.2 Attributions 1.2.1 Advisors 1.2.2 Team 1.2.3 Others

Team University of Texas at Austin

The University of Texas is the flagship public university of the UT system and resides in Austin, Texas. It's a fun place to live and a great place to do science.

Who we are

Advisor: Prof. Jeff Barrick Grad students: Jared Ellefson Michael Hammerling Erik Quandt Undergrads: Razan Alnahhas Logan Bachman Will Darden Aurko Dasgupta Peter Otoupal Ben Slater

Jeffrey barrick.jpg Jeff Barrick; Assistant Professor of Chemistry and Biochemistry Jared.jpg Jared Ellefson; Man with the Plan and part time Blues Brother Michael hammerling.jpg Michael Hammerling; second year Graduate student in Cell and Molecular Biology Erik quandt.jpg Erik Quandt; Graduate student in Cell and Molecular Biology Razan.jpg Razan Alnahhas: third year undergraduate studying human biology Logan Bachman.jpg Logan Bachman; third year undergraduate biochemistry major, also awesome Will Darden.png Will Darden: third year undergraduate studying Computational Biology Aurko pretending to be a scientist.jpg Aurko Dasgupta: fourth year Cell and Molecular Biology student Peter Otoupal Doing Science Stuff.jpg Peter Otoupal ; fourth year Chemical Engineering undergraduate, with a penchant for debauchery Ben Slater: Undergrad in junior year; studying cellular and molecular biology

Attributions

Advisors

• Prof. Jeff Barrick - faculty sponsor. A little graphic design for the website and coordinated getting Pseudomonas strain for Caffeinated coli. Found numbers to do calculation of number of guanines in E. coli.
• Jared Ellefson - Main ZombiE.coli project advisor.
• Michael Hammerling - Main Spinach-mCherry dual reporter project advisor. Advised students on lab work for ZombiE.coli and Caffeinated coli.
• Erik Quandt - Main caffeinated coli project advisor.

Team

• Razan Alnahhas -Constructed and tested pConverter.
• Logan Bachman - Constructed and tested Spinach and Spinach-mCherry reporters.
• Will Darden - Performed simulations of ZombiE.coli. Analysis of whole-genome shotgun sequencing of Pseudomonas putida CBB5.
• Aurko Dasgupta - Human practices, safety officer.
• Peter Otoupal - Assayed Caffeinated Coli constructs built by Ben. Performed caffeinated beverage and caffeine growth tests for decaffeination operon.
• Ben Slater - Constructed and tested pReporter and pReporter.permaflipped. Designed website's CSS stylesheet, navbar, and header. Assisted Peter and Erik with Caffeinated coli assays and construction.

Others

• [http://www.engineering.uiowa.edu/cbe/faculty-staff/mani-v-subramanian Prof. Mani Subramanian and Ryan Summers of the University of Iowa] for generously sharing the Pseudomonas putida CBB5 strain with us.
• [http://openwetware.org/wiki/User:Xi_Che Xi Chen of the Ellington Lab] for the original pET plasmid containing the spinach aptamer.
• [http://openwetware.org/wiki/CH391L/S12 Colleagues in the Spring 2012 CH 391L Synthetic Biology class] at UT Austin for ideas and research that inspired these projects.