Team:Groningen/Labwork/30 July 2013
From 2013.igem.org
Mirjam
Started gel extraction for the restricted backbone+promoter, RFP and eYFP. RFP and eYFP are dissolved in 30µl elution buffer, to get a higher concentrationRun a 0.8% agarose gel to examine the restriction. Gel revealed only a very small band for RFP.
Did a nanodrop on the samples to examine their concentration. Concentration of the backbone is 0.8 ng/µl, RFP 6.4 ng/µl and eYFP 2.4 ng/µl.