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Team:Groningen/Labwork/31 July 2013
From 2013.igem.org
Mirjam
Examined the plates of eYFP and GFP grown on Lb agar + cm plates. This reveals that there are cells growing and the negative control did not show any colonies. Six colonies per type are inoculated in LB medium with cm to perform a mini prep later on.Examined the plates of the ligation product BBa_k823823+LacI with eYFP and GFP. These plates did not show any colonies, although the negative control did, which is very strange.
The plated B.subtilis strain did grow. So three single colonies are inoculated to perform a transformation reaction.
iGEM 2013 Groningen
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