Team:Groningen/Labwork/1 August 2013

From 2013.igem.org

Revision as of 06:59, 1 August 2013 by Mirjam (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Mirjam

Plates for the transformation of ΔDes,ΔCheY and ΔDes/ΔCheY did not show any colonies. The plates for eYFP showed some colonies on the plates with the transformation to E.coli. The plates with RFP and the ligation of Pdes/Chey did not show any colonies. Also the plates with a transformation of GFP240 and GFP0840 showed colonies.
Inoculated the colonies into LB with ampicillin to perform a miniprep later on
Made a new restriction digestion for RFP, eYFP and BBa_k823823 + LacI with EcoRI and PstI.