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From 2013.igem.org

Restriction Digest Protocol

Protocol can be found at: http://parts.igem.org/Help:Protocols/Restriction_Digest

Materials

  -Ice and bucket/container
  -(1) 8-tube strip, or (3) 0.6ml thin-walled tubes
  -Part A (Purified DNA, > 16ng/ul)
  -Part B (Purified DNA, > 16ng/ul)
  -Linearized plasmid backbone (25ng/ul)
  -dH2O
  -NEB Buffer 2, 3.1,or Ecor1
  -BSA (Not needed with 3.1 buffer)
  -Restriction Enzymes: EcoRI, SpeI, XbaI, PstI, DpnI
  -Thermal cycler 

Procedure