12/08/13

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Sonicating herring sperm DNA

  • Pipetting out sonicated samples every 30mins
    • Pipette 4ul of DNA into Son 1-6 every 30mins
      • Dilute 1ul of DNA from Son 1-6 in 9ul of 1xTE buffer to tubes 10xdil 1-6
      • Vortex each dilution thoroughly and centrifuge briefly.
  • Keep tubes on ice

Running an agarose gel for sonication analysis

  • To tubes 10xdil 1-6, add 5ul of sterile water and 5ul of orange G dye. Centrifuge briefly. Keep on ice until loading.
  • Use 5ul of 1kb marker on one end, and 5ul of 100kb marker on other end.
  • Add buffer so that wells in the tray and the tray itself are covered
  • Run at 100V for an hour
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