Description

The aim of the experiment was to characterize the behavior of wild type E.coli in anaerobic conditions in terms of the conversion of nitrite to ammonium.

The experiment was performed by adding three different concentrations of nitrite to a medium with a growing native strain of E. coli. The concentrations of NH₄⁺ and NO₂^- were measured every 20 minutes and the experiment lasted for 3 hours. Also three controls with biomass only, without biomass and killed biomass were analyzed in order to exclude any external influences on the nitrite conversion.

Methods

Protocol [1]

Results

Mg of Nitrogen (as Nitrite or Ammonia) per litre measured for varying concentrations of Nitrite added at t=0. All experiments were performed anaerobically. Samples were measured every 20 min for 3 hours.